Biomedical Nanotechnology

Methods and Protocols

Author: Sarah J. Hurst

Publisher: Humana Press

ISBN: 9781617790515

Category: Science

Page: 436

View: 675

Due to their unique size-dependent properties, nanomaterials have the potential to revolutionize the detection, diagnosis, and treatment of disease by offering superior capabilities compared to conventionally-used materials. Biomedical Nanotechnology: Methods and Protocols brings together experts from a wide variety of fields to provide a practical overview of biomedical nanotechnology, from the conception of novel materials in the laboratory to the application of such structures in the clinic. After a brief introductory chapter, the first section consists of protocol chapters which provide hands-on information on the synthesis of a variety of solution-phase and surface-bound nanomaterials and their application in sensing, imaging, and/or therapeutics, while the second section consists of a series of case studies and review chapters that discuss the toxicology of nanomaterials, the regulatory pathways to US Food and Drug Administration (FDA) approval of these materials, their patenting, marketing, and commercialization, and the legal and ethical issues surrounding their use. Written in the highly successful Methods in Molecular BiologyTM series format, many chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible protocols, and insightful tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Biomedical Nanotechnology: Methods and Protocols surveys this exciting field from the most vital angles in order to provide a comprehensive reference for scientists and researchers of all different backgrounds looking to utilize the numerous versatile applications of nanomaterial technologies.

Cancer Nanotechnology

Methods and Protocols

Author: Stephen R. Grobmyer,Brij M. Moudgil

Publisher: Humana Press

ISBN: 9781607616139

Category: Medical

Page: 366

View: 9693

Early detection of cancer at the cellular level, even before anatomic anomalies are visible, is critical to more efficacious and cost effective diagnosis and therapeutic advances. In Cancer Nanotechnology: Methods and Protocols, an international panel of experts provide the most recent, cutting-edge, "how-to" approaches developed and employed by researchers in a variety of disciplines to identify cancer specific biomarkers, construct suitable multifunctional targeted nanostructure platforms, along with enhanced imaging and therapeutic applications. Covering such topics as multifunctional and multimodal nanoparticles, nanoparticle mediated cancer theranostics, molecular targets for cancer nanotechnology, and nanoparticles for non-invasive image-guided cancer therapy, the volume addresses the key challenges of the field today, specifically targeted and localized delivery of the drugs. As a volume in the highly successful Methods in Molecular BiologyTM series, the protocols chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cancer Nanotechnology: Methods and Protocols integrates cancer biology, clinical oncology, molecular cancer imaging, materials science and chemical engineering, biomedical engineering, toxicology, computer science, electrical engineering, chemistry, physics, and mathematics in order to achieve the vital goals of nanotechnology-mediated early cancer detection and more efficacious and less toxic therapies for these devastating diseases.

Nanomaterial Interfaces in Biology

Methods and Protocols

Author: Paolo Bergese,Kimberly Hamad-Schifferli

Publisher: Humana Press

ISBN: 9781627034616

Category: Science

Page: 293

View: 8477

The intersection of nanotechnology with biology has given rise to numerous ideas for new ways to use nanotechnology for biological applications. Nanomaterials possess unique size- and material-dependent properties which make them attractive for improving regular biomedical fields, such as drug delivery, imaging, therapy, and diagnostics. Divided into three convenient sections, Nanomaterial Interfaces in Biology: Methods and Protocols covers protocols describing synthesis, fabrication, and construction of bio-nanomaterial interfaces, characterization protocols of bio-nanomaterial interfaces, and applications which utilize the bio-nanomaterial interfaces. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible Nanomaterial Interfaces in Biology: Methods and Protocols will serve the new and emerging scientific community, enabling new capabilities and technologies that were not previously possible in medicine and biology.

Cancer Nanotechnology

Methods and Protocols

Author: Reema Zeineldin

Publisher: Humana Press

ISBN: 9781493966448

Category: Medical

Page: 490

View: 5613

This detailed volume presents protocols for advancing the utility of nanotechnology in cancer research toward improving our understanding of cancer biology, prevention, diagnosis, and therapy. There are continuous new discoveries in the field of nanotechnology, thus creating new imaging systems or therapies, and this book focuses on how to employ certain discoveries for studying cancer by presenting principles along with techniques to allow for the transformation of any new discoveries in the field into cancer-studying tools with the hope of bringing in the involvement of biomedical scientists who can enhance the speed of discoveries toward cancer diagnosis and therapy. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and motivating, Cancer Nanotechnology: Methods and Protocols serves as an ideal resource for biomedical scientists interested in the potential of this field as well as for physical scientists and engineers interested in employing nanotechnology in cancer diagnosis and therapy./div

DNA Nanotechnology

Methods and Protocols

Author: Giampaolo Zuccheri

Publisher: N.A

ISBN: 9781493985821

Category: Biotechnology

Page: 334

View: 9082

This second edition provides new and updated methods that detail new DNA nanotechnology techniques. Chapters focus on DNA origami nanostructures for arranging matter in the nanoscale or on their manipulation with the aid of other technologies, on procedures for making nucleic acids nanostructures of different kinds, and methods to simulate complex nanostructures or to use them in biosensing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, DNA Nanotechnology: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.

Nanotechnology in Regenerative Medicine

Methods and Protocols

Author: Melba Navarro,Josep A. Planell

Publisher: Humana Press

ISBN: 9781493961788

Category: Science

Page: 319

View: 1032

Nanotechnology plays a key leading role in developing tools able to identify, measure, and study cellular events at the nanometric level as well as in contributing to the disclosure of unknown biological interactions and mechanisms, which opens the door for advances including nanodevices for diagnostic and therapy, drug delivery systems, and regenerative medicine. In Nanotechnology in Regenerative Medicine: Methods and Protocols, expert researchers in the field provide an overview of a very wide range of currently used technologies and methods that involve nanotechnology principles applicable to tissue regeneration. Being that the application of nanotechnology to regenerative medicine is a very broad field, this book focuses its interests on particular areas such as its use as a means to produce efficient platforms and structures for tissue engineering, delivery systems and biosensors, as well as the use of some techniques to study materials surfaces and the interactions between cells, biomolecules, and surfaces at the nanoscale. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their related topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Nanotechnology in Regenerative Medicine: Methods and Protocols provides established scientists, junior researchers, and students involved in the bioengineering, biotechnology, and biomedical fields with a sound foundation in a variety of vital nanotechnology approaches in regenerative medicine.

RNA Nanotechnology and Therapeutics

Author: Peixuan Guo,Farzin Haque

Publisher: CRC Press

ISBN: 1466505664

Category: Medical

Page: 611

View: 7095

Interest in RNA nanotechnology has increased in recent years as recognition of its potential for applications in nanomedicine has grown. Edited by the world's foremost experts in nanomedicine, this comprehensive, state-of-the-art reference details the latest research developments and challenges in the biophysical and single molecule approaches in RNA nanotechnology. In addition, the text also provides in-depth discussions of RNA structure for nanoparticle construction, RNA computation and modeling, single molecule imaging of RNA, RNA nanoparticle assembly, RNA nanoparticles in therapeutics, RNA chemistry for nanoparticle synthesis, and conjugation and labeling.

Liposomes

Methods and Protocols

Author: Volkmar Weissig

Publisher: N.A

ISBN: N.A

Category: Liposomes

Page: N.A

View: 1311


Nanotechnology in Biology and Medicine

Methods, Devices, and Applications

Author: Tuan Vo-Dinh

Publisher: CRC Press

ISBN: 9781420004441

Category: Science

Page: 792

View: 4288

The combination of biology and nanotechnology has led to a new generation of nanodevices that make it possible to characterize the chemical, mechanical, and other molecular properties, as well as discover novel phenomena and biological processes occurring at the molecular level. These advances provide science with a wide range of tools for biomedical applications in therapeutic, diagnostic, and preventive medicine. Nanotechnology in Biology and Medicine: Methods, Devices, and Applications integrates interdisciplinary research and recent advances in instrumentation and methods for applying nanotechnology to various areas in biology and medicine. Pioneers in the field describe the design and use of nanobiosensors with various analytical techniques for the detection and monitoring of specific biomolecules, including cancer cells. The text focuses on the design of novel bio-inspired materials, particularly for tissue engineering applications. Each chapter provides introductory material including a description of methods, protocols, instrumentation, and applications, as well as a collection of published data with an extensive list of references. An authoritative reference written for a broad audience, Nanotechnology in Biology and Medicine: Methods, Devices, and Applications provides a comprehensive forum that integrates interdisciplinary research to present the most recent advances in protocols, methods, instrumentation, and applications of nanotechnology in biology and medicine.

Nanostructure Design

Methods and Protocols

Author: Ehud Gazit,Ruth Nussinov

Publisher: Springer Science & Business Media

ISBN: 9781934115350

Category: Science

Page: 267

View: 2524

As one of the fastest growing fields of research in the 21st century, nanotechnology is sure to have an enormous impact on many aspects of our lives. Nanostructure Design: Methods and Protocols serves as a major reference for theoretical and experimental considerations in the design of biological and bio-inspired building blocks, the physical characterization of the formed structures, and the development of their technical applications. The chapters contributed by leading experts are divided into two sections, the first of which covers experimental aspects of nanostructure design and the second delves into computational methods. As a volume of the highly successful Methods in Molecular BiologyTM series, this collection pulls together cutting-edge protocols, written in a step-by-step, readily reproducible format certain to guide researchers to the desired results. Comprehensive and essential, Nanostructure Design: Methods and Protocols uses biological principles and vehicles on design to aid scientists in the great challenges still ahead.

Oxidative Stress and Nanotechnology

Methods and Protocols

Author: Donald Armstrong,Dhruba J Bharali

Publisher: Humana Press

ISBN: 9781627034746

Category: Science

Page: 294

View: 766

The emergence of nanotechnology has had a profound effect on almost every aspect of the 21st century’s daily life. It has had a revolutionary impact from stain-resistant clothing and cosmetics to environmental issues , including energy and medicine and even aerospace engineering. In Oxidative Stress and Nanotechnology: Methods and Protocols, expert researchers in the field detail various aspects of nanotechnology from the oxidative stress point of view. Focusing on synthesis of different antioxidant nanoparticles and antioxidant-loaded nanoparticles, as well as their in vitro/ in vivo mechanisms of action along with their clinical relevance. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Oxidative Stress and Nanotechnology: Methods and Protocols seeks to aid scientists in the further study of techniques for nanotechnology in pathophysiology and protection from the oxidative stress point of view.

Pattern Recognition in Computational Molecular Biology

Techniques and Approaches

Author: Mourad Elloumi,Costas Iliopoulos,Albert Y. Zomaya,Jason T. L. Wang

Publisher: John Wiley & Sons

ISBN: 1118893689

Category: Computers

Page: 656

View: 4669

A comprehensive overview of high-performance pattern recognition techniques and approaches to Computational Molecular Biology This book surveys the developments of techniques and approaches on pattern recognition related to Computational Molecular Biology. Providing a broad coverage of the field, the authors cover fundamental and technical information on these techniques and approaches, as well as discussing their related problems. The text consists of twenty nine chapters, organized into seven parts: Pattern Recognition in Sequences, Pattern Recognition in Secondary Structures, Pattern Recognition in Tertiary Structures, Pattern Recognition in Quaternary Structures, Pattern Recognition in Microarrays, Pattern Recognition in Phylogenetic Trees, and Pattern Recognition in Biological Networks. Surveys the development of techniques and approaches on pattern recognition in biomolecular data Discusses pattern recognition in primary, secondary, tertiary and quaternary structures, as well as microarrays, phylogenetic trees and biological networks Includes case studies and examples to further illustrate the concepts discussed in the book Pattern Recognition in Computational Molecular Biology: Techniques and Approaches is a reference for practitioners and professional researches in Computer Science, Life Science, and Mathematics. This book also serves as a supplementary reading for graduate students and young researches interested in Computational Molecular Biology. Mourad Elloumi, PhD, is Professor in Computer Science at the University of Tunis-El Manar, Tunisia. Dr. Elloumi is the author/co-author of more than 50 publications in international journals and conference proceedings related to Algorithmics, Computational Molecular Biology, and Knowledge Discovery and Data Mining. Costas S. Iliopoulos, PhD, is Professor of AlgorithmDesign at King's College London, UK. Dr. Iliopoulos co-authored over 300 peer-reviewed articles in pattern matching and combinatorics of strings. He serves on the editorial board of the Journal of Discrete Algorithms, Computer Mathematics & Combinatorial Computing, and System Biology & Biomedical Technologies. Jason T. L. Wang, PhD, is Professor of Computer Science at the New Jersey Institute of Technology, USA. Dr. Wang has published extensively on Data Mining and Computational Molecular Biology, and has been a member of program committees for over 200 conferences and workshops in these and related areas. Albert Y. Zomaya, PhD, is the Chair Professor of High Performance Computing & Networking in the School of Information Technologies, University of Sydney, Australia. Dr. Zomaya published more than 500 scientific papers and articles and is author, co-author or editor of more than 20 books. Dr. Zomaya is Fellow of AAAS, IEEE, and IET.

Stem Cell Nanotechnology

Methods and Protocols

Author: Kursad Turksen

Publisher: Humana Press

ISBN: 9781493963270

Category: Science

Page: 184

View: 5989

Stem Cell Nanotechnology: Methods and Protocols gathers several representative protocols related to the emerging interest in nanotechnology as it relates to stem cell biology. The detailed chapters presented within have been validated for reproducibility and are described in an easy to follow, step-by-step fashion so as to be valuable for not only experts but also novices in the stem cell field. As with other volumes in the highly successful Methods in Molecular Biology series, chapters conclude with a Notes section, which provides tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Stem Cell Nanotechnology: Methods and Protocols provides both a flavor of the field as it currently is and a source to stimulate new approaches and methodologies by those interested in nanotechnological applications.

Nanotechnology for Nucleic Acid Delivery

Methods and Protocols

Author: Manfred Ogris,David Oupicky

Publisher: Humana Press

ISBN: 9781627031394

Category: Science

Page: 286

View: 2316

Nanotechnology and nucleic acid based therapies are two emerging fields in science whose combination has the potential to improve quality of life for patients suffering from various diseases that can so far only be treated in an unsatisfactory way. Nucleic acids offer the potential for highly selective treatment of such diseases or the highly specific modulation of gene expression with RNA interference. A key issue for successful nucleic acid therapies is the availability of a suitable delivery system. Here, the field of nanotechnology offers a multitude of possibilities to develop nanosized delivery vectors tailor-made for various local and systemic approaches. In Nanotechnology for Nucleic Acid Delivery: Methods and Protocols, experts in the field cover the area of nanoparticulate delivery of nucleic acids in terms of biosafety, particle synthesis as well as its application in cell culture. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Nanotechnology for Nucleic Acid Delivery: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies.

The Art of Writing Reasonable Organic Reaction Mechanisms

Author: Robert B. Grossman

Publisher: Springer Science & Business Media

ISBN: 0387954686

Category: Science

Page: 360

View: 8923

Intended for students of intermediate organic chemistry, this text shows how to write a reasonable mechanism for an organic chemical transformation. The discussion is organized by types of mechanisms and the conditions under which the reaction is executed, rather than by the overall reaction as is the case in most textbooks. Each chapter discusses common mechanistic pathways and suggests practical tips for drawing them. Worked problems are included in the discussion of each mechanism, and "common error alerts" are scattered throughout the text to warn readers about pitfalls and misconceptions that bedevil students. Each chapter is capped by a large problem set.

Nanocarriers for Drug Delivery

Nanoscience and Nanotechnology in Drug Delivery

Author: Shyam Mohapatra, MD,Shivendu Ranjan,Nandita Dasgupta,Raghvendra Kumar Mishra,Sabu Thomas

Publisher: Elsevier

ISBN: 9780128140338

Category: Science

Page: 680

View: 4351

Nano-carriers for Drug Delivery: Nanoscience and Nanotechnology in Drug Delivery presents recent discoveries in research on the pharmaceutical applications of the various types of nanosystem-based drug delivery systems. As many nanosystems have reached the market over the past decade, this book proves their benefits to patients. It explores these new carriers and the advances in drug delivery they have facilitated. Reflecting the interdisciplinary nature of the subject matter, the book includes experts from different fields, and with various backgrounds and expertise. It will appeal to researchers and students from different disciplines, such as materials science, technology and various biomedical fields. Coverage includes industrial applications that bridge the gap between lab-based research and practical industrial use. The resulting work is a reference and practical source of guidance for researchers, students and scientists working in the fields of nanotechnology, materials science and technology and biomedical science. Enables readers from different fields to access recent research and protocols across traditional boundaries Focuses on protocols and techniques, as well as the knowledge base of the field, thus enabling those in R&D to learn about, and successfully deploy, cutting-edge techniques Includes sections on nanocarrier systems

Bioluminescence Methods and Protocols

Author: Robert A. LaRossa

Publisher: Springer

ISBN: 1592595774

Category: Bioluminescence assay

Page: 310

View: 2447

Comprehensive and state-of-the-art, Bioluminescence Methods and Protocols brings together a collection of innovative methods for the use of luciferase and green fluorescent protein in analytical biochemistry, molecular toxicology, environmental monitoring, and cell-based assays. The contributors-all hands-on researchers who have perfected their protcols-illustrate these powerful methods with a wide variety of applications ranging from the analytical determination (titer) of specific molecules to the cellular detection of a wide range of pollutants, to microbiological detection and analysis, and the molecular biological manipulation of lux, luc, and gfp structural genes. Step-by-step methods for the construction of other recombinant organisms are also included. Bioluminescence Methods and Protocols represents the state-of-the-art in luminescent assays. Its cutting-edge techniques, diversely illustrated applications, and detailed instructions will make it the standard reference for today's investigators who want to adapt established techniques to their purposes or apply them to a wide range of clinical, microbiological, environmental, toxicological, and analytical problems.

Bioconjugation Protocols

Strategies and Methods

Author: Christof M. Niemeyer

Publisher: Springer Science & Business Media

ISBN: 1592598137

Category: Medical

Page: 330

View: 1905

Expert laboratorians update the classic bioconjugation methods and introduce valuable new techniques that go beyond pure biomedical applications to include elements from advanced organic synthesis, molecular biology, and materials science. These readily reproducible methods cover the preparation of protein conjugates using covalent and noncovalent conjugation, the synthesis of nucleic acid conjugates using a variety of labeling techniques, and approaches to semisynthetic conjugates of proteins. Additional chapters address the biofunctionalization of inorganic surfaces, including the on-chip synthesis of peptide nucleic acids to generate microarrays for the high-throughput analysis of RNA and DNA, gold nanaoparticles, and carbon nanotube probes for atomic force microscopy.